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h9 human escs  (WiCell Research Institute Inc)


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    WiCell Research Institute Inc h9 human escs
    Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human <t>ESCs</t> <t>(H9-EOS).</t> AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .
    H9 Human Escs, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 3811 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    h9 human escs - by Bioz Stars, 2026-06
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    1) Product Images from "AMPK-p38 axis converts human pluripotent stem cells to naive state"

    Article Title: AMPK-p38 axis converts human pluripotent stem cells to naive state

    Journal: iScience

    doi: 10.1016/j.isci.2026.115569

    Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human ESCs (H9-EOS). AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .
    Figure Legend Snippet: Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human ESCs (H9-EOS). AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .

    Techniques Used: Flow Cytometry, Expressing, Quantitative RT-PCR, Immunostaining, Fluorescence, Microscopy



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    Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human <t>ESCs</t> <t>(H9-EOS).</t> AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .
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    Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human <t>ESCs</t> <t>(H9-EOS).</t> AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .
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    Image Search Results


    Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human ESCs (H9-EOS). AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .

    Journal: iScience

    Article Title: AMPK-p38 axis converts human pluripotent stem cells to naive state

    doi: 10.1016/j.isci.2026.115569

    Figure Lengend Snippet: Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human ESCs (H9-EOS). AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .

    Article Snippet: Primed H9 human ESCs were obtained from WiCell (WA09).

    Techniques: Flow Cytometry, Expressing, Quantitative RT-PCR, Immunostaining, Fluorescence, Microscopy

    (a) Transcriptomic analysis of the time-course differentiation process. Loss of pluripotency markers is visible over time. (b) A GM25256 hiPSC colony after 12 h of RA-induced differentiation. The red arrow indicates filopodium-like membrane protrusion at colony periphery. The green arrow indicates an intercellular gap. (c) A GM25256 hiPSC colony after 24 h of RA-induced differentiation. (d) A GM25256 hiPSC colony after 48 h of RA-induced differentiation. The red arrow indicates a jagged colony boundary. ( e ) A GM25256 hiPSC colony after 96 h of RA-induced differentiation. The red arrow indicates an intercellular gap near the colony periphery. ( f ) A H9 hESC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (g) Ratio of H9 hESC colonies with average P undiff over 0.5. (h) A KOLF2.1J hiPSC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (i) Ratio of KOLF2.1J hiPSC colonies with average P undiff over 0.5. Scale bar (B to H) = 50 μm.

    Journal: bioRxiv

    Article Title: Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency Exit

    doi: 10.64898/2026.04.23.720508

    Figure Lengend Snippet: (a) Transcriptomic analysis of the time-course differentiation process. Loss of pluripotency markers is visible over time. (b) A GM25256 hiPSC colony after 12 h of RA-induced differentiation. The red arrow indicates filopodium-like membrane protrusion at colony periphery. The green arrow indicates an intercellular gap. (c) A GM25256 hiPSC colony after 24 h of RA-induced differentiation. (d) A GM25256 hiPSC colony after 48 h of RA-induced differentiation. The red arrow indicates a jagged colony boundary. ( e ) A GM25256 hiPSC colony after 96 h of RA-induced differentiation. The red arrow indicates an intercellular gap near the colony periphery. ( f ) A H9 hESC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (g) Ratio of H9 hESC colonies with average P undiff over 0.5. (h) A KOLF2.1J hiPSC colony both untreated and after 24 h of RA-induced differentiation. P undiff was measured by DeepHOPE. (i) Ratio of KOLF2.1J hiPSC colonies with average P undiff over 0.5. Scale bar (B to H) = 50 μm.

    Article Snippet: The human embryonic stem cell line H9 (WA09, WiCell) and human iPSCs lines GM25256 (Coriell Institute) and KOLF2.1J (The Jackson Laboratory) were used to generate the base model for this study.

    Techniques: Membrane